9/11/2023 0 Comments Perfect split c4d downloadAll cases negative by immunofluorescence were also negative by immunohistochemistry. How-ever, immunohistochemistry staining caused more dif-ficulties in interpretation, due to nonspecific staining in tubular cells and surrounding interstitium. There was total classification agreement between methods in 87.4% (125/143) of cases. For this purpose complement 4d staining was performed retrospectively by the two methods in indication biopsies (n=143) and graded using the Banff 07 classification. We describe the advantages and disadvantages of the immunohistochemistry vs. The aim of this study is to evaluate the sensitivity and specificity of immunohis-tochemistry compared with immunofluorescence. The immunohistochemistry technique applied to paraffin-embedded tissue may be used when no frozen tissue is available. The immu-nofluorescence technique applied to frozen sections is the present gold-standard method for complement 4d staining and is used routinely in our laboratory. Peritubular capillary complement 4d staining is one of the criteria for the diagnosis of antibody-mediated rejection, and research into this is essential to kidney allograft evaluation.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |